Elucidation of mechanism of neuroendocrine control in reproduction for advancement of artificial seed production of commercial bivalve mollusks

我们克隆了扇贝(PyGnRH)和牡蛎(CgGnRH)中枢神经系统促性腺激素释放激素前体(GnRH)样多肽的cDNA转录产物。我们成功地从两种贻贝和日本扇贝中克隆了两种新的雌激素受体。这些受体分为雌激素受体(ER)和雌激素相关受体(ERR)，在卵巢和睾丸中均有表达。比目鱼Era介导的基因分析表明，Pyer是雌激素的转录因子，而Pyer是结构性激活剂。5-羟色胺(5-HT)诱导的卵母细胞成熟和精子运动被卵母细胞成熟抑制因子(OMAF)抑制，这是由于细胞外钙内流的干扰。抑制的OMAF由于被抗体吸收而释放，放大了5-羟色胺诱导的产卵。

We cloned the cDNA transcripts of prepro Gonadotropin Releasing Hormones (GnRH)-like peptides from the central nervous system of scallop(pyGnRH)and oyster (cgGnRH). The putative pyGnRH peptide stimulated the proliferation of spermatogonia, which was mediated through the induction of estrogen synthesis by pyGnRH.We successfully cloned two novel forms of estrogen receptor from two kinds of mussels and Japanese scallop. These receptors were classified each into an estrogen receptor (ER) and estrogen-related receptor (ERR) and were expressed in both ovary and testis. The flounder ERa-mediated gene assay suggested that pyER behaves as a transcription factor to estrogen while pyERR is a constitutive activator.Serotonin (5-HT)-induced oocyte maturation and sperm motility is inhibited by oocyte maturation arresting factor (OMAF) due to interference of extracellular Ca2+ influx. The release from suppressive OMAF due to absorption with antibody against OMAF amplified the 5-HT-induced spawning.