Central project Gene Expression and Proteome Dynamics in Chlamydomonas reinhardtii

所有项目都涉及单细胞绿藻莱茵衣藻，由于其具有与酵母相似的优势，因此在过去几年中作为真核模型系统出现。由于这个原因，这种藻类的基因组计划已经在日本和美国启动，并且进展非常迅速。目前，整个叶绿体和线粒体基因组已被测序。目前有超过136,000种est可用，整个核基因组目前由美国能源部进行测序。许多可用的方法和创新程序的进一步发展为研究特定科学问题的基因表达和蛋白质组动力学提供了新的可能性。研究小组旨在以合作的方式利用这一潜力，以调查这些方面。因此，重点将放在选定的主题上，这些主题特别适合C. reinhardtii作为模型系统。这些包括叶绿体的生物发生和功能及其与细胞核的相互作用，基底器官的组成和功能以及调节策略过程的生物钟。因此，莱因哈蒂梭菌对人类的某些蛋白质（如鞭毛/基础器官，昼夜节律系统）的保护提供了研究这种藻类的示范过程并将知识转移到哺乳动物系统的可能性。此外，还将研究选择基因的控制过表达、基因沉默、转基因在基因组内的位置效应、染色质重塑因子的表征及其对外源基因表达的影响等新的分子机制。与拟南芥（Arabidopsis thaliana）等高等植物模型系统相比，这种藻类可以轻松快速地大量生长，这对所有基于蛋白质组的项目都是高度相关的。因此，它为识别通常在细胞中最低限度存在的调节因子提供了极好的条件。这些优势将被用于叶绿体（类核、rna结合蛋白、光系统I组装）、昼夜节律调节系统（时钟控制的核酸结合蛋白和磷酸化蛋白）和基底体内的功能蛋白质组学。为此，我们希望建立一个蛋白质组学中心，为所有蛋白质组学工具提供一个平台。

All projects are dealing with the unicellular green alga Chlamydomonas reinhardtii, which emerged as eukaryotic model system during the past years since it offers similar advantages than yeast. For this reason, genome projects of this alga have been initiated in Japan as well as in the USA and have proceeded very rapidly. Currently, the entire chloroplast and mitochondria genomes have been sequenced. There are more than 136,000 ESTs available and the entire nuclear genome is presently sequenced by the Department of Energy in the USA. The numerous methods available and the further development of innovative procedures offer new possibilities for studying the gene expression and proteome dynamics of specific scientific questions. The research group aims to use this potential in a cooperative manner in order to investigate these aspects. Thereby, the focus will be on selected topics, which are especially suitable with C. reinhardtii as a model system. These include the biogenesis and function of the chloroplast and its interaction with the nucleus, the composition and function of the basal apparatus and of the circadian clock regulating tactic processes. Thereby, the conservation of certain proteins from C. reinhardtii to humans (e.g. flagella/basal apparatus, circadian system) offers the possibility to study exemplary processes in this alga and transfer the knowledge to the mammalian system. In addition, new molecular mechanisms of controlled overexpression of selected genes, gene silencing, position effects of transgenes within the genome, the characterization of chromatin remodeling factors and their influence on foreign gene expression will be worked out. For all proteome based projects it is highly relevant that this alga, in contrast to higher plant model systems like Arabidopsis thaliana, can be easily and quickly grown in large quantities. Therefore, it offers excellent conditions for identifying regulatory factors, which are often minimally present in cells. These advantages will be used to apply functional proteomics within the chloroplast (nucleoid, RNA-binding proteins, photosystem I assembly), the circadian regulatory system (clock-controlled nucleic acid-binding- and phospo-proteins) and the basal body. For this purpose, we want to establish a proteome center that provides a platform for all proteomic tools.