structure and function of mitochondria at presynaptic terminal

线粒体的功能对活细胞的各个方面都至关重要。它的形态特征对活细胞动力学，特别是能量供应也很重要。我将重点放在突触前线粒体上，因为突触前末端是由神经元活动控制的囊泡交通的专用机器。首先，我检查了突触前线粒体是否自由移动。FCS分析表明，著名的微管干扰剂诺可达唑影响了突触前线粒体的稳定性。笔者认为，与微管的连接可能确保了线粒体突触前能量供应的活性依赖性。为了解决这个问题，我开发了新的cAMP探针。它可以可视化线粒体内cAMP的动态。[cAMP]mt被认为是电子传递活性的重要调制剂。我提出了一个假设，即突触前活动诱导[cAMP]mt的增加，这取决于微管平台，然后上调ATP的产生，用于突触传递的能量使用。

Mitochondrial function is essential for various aspects of living cell. Its morphological feature should be also important for living cell dynamics especially for the energy supply. I focus on the presynaptic mitochondria because presynaptic terminal is the specialized machine in terms of vesicular traffic which is controlled by the activity of neuron. First of all, I checked that presynaptic mitochondria is free moving or not. FCS analysis revealed that nocodazole, famous microtubule disrupting reagent, has affected the presynaptic mitochondrial stability. I conclude that the linking with microtubule might ensure the activity dependency of presynaptic energy supply from the mitochondria. To address this issue, I developed the novel cAMP probe. It can visualize the cAMP dynamics inside the mitochondria. It has been believed that [cAMP]mt is important modulator for electron transport activity. I have proposed the hypothesis that presynaptic activity induce the [cAMP]mt increase, which depend on microtubule platform, then up-regulate ATP production for energy use of synaptic transmission.