Development of a method for simultaneous detection of 4 opportunistic pathogen in mouse

很少在免疫正常的小鼠中引起感染的机会性生物，可以在免疫功能低下的小鼠中引起感染。由于机会性感染可能会改变免疫功能受损小鼠的研究结果，因此证明免疫功能受损小鼠群体不含特定机会性病原体的健康证明对生物医学研究人员很重要。本研究旨在建立一种简单、快速的环介导等温扩增（LAMP）诊断方法，用于检测4种条件致病菌：金黄色葡萄球菌（Sa）、小鼠肝炎病毒（MHV）、小鼠诺如病毒（MNV）和肺性巴氏杆菌（Pp）。用于检测MHV和MNV的逆转录- lamp方法已经开发并发表在《病毒学方法杂志》上。LAMP法也被用于检测耐防腐剂和耐甲氧西林的Sa。然而，用于检测Pp的LAMP方法仍在建设中。

Opportunistic organisms, which rarely cause infections in immunocompetent mice, can cause infections in immunocompromised mice. Since opportunistic infection may alter research results using immunocompromised mice, health certificate showing the immunocompromised mouse colony is free from specific opportunistic pathogens is important for biomedical researchers.The purpose of this study is development of simple and rapid diagnostic loop-mediated isothermal amplification (LAMP) methods for detecting 4 opportunistic pathogens: Staphylococcus aureus (Sa), mouse hepatitis virus (MHV), murine norovirus (MNV), and Pasteurella pneumotropica (Pp). Reverse transcription-LAMP methods for the detection of MHV and MNV have been developed and published in the Journal of Virological Methods. LAMP methods for the detection of antiseptic- and methicillin-resistant Sa had also been developed. However, LAMP method for the detection of Pp has been under construction.