Development of a novel combinatorial synthetic receptor library using triple strand DNA

本研究旨在构建一个新的组合合成受体文库，在该文库中，三个随机序列的多肽连接到一个三链DNA上。为了构建文库，氨基酸和相应的DNA标签被连接到三链DNA的每个单链DNA的末端。这使得能够通过多价结合来构建对靶分子具有高选择性和亲和力的文库。此外，多肽的序列可以通过DNA标签来确定。我尝试用三个氨基酸构建了一个双肽文库，并成功地将这些氨基酸和相应的DNA标签与单链DNA连接，形成了三链DNA。使用这个库的选择现在正在计划中。

This study aims to construct a novel combinatorial synthetic recepter library in which three peptides with random sequencse are linked to a triple strand DNA. To constract the library, amino acids and corresponding DNA tags are linked to the ends of each ssDNA of the triple strand DNA. This enables the construction of a library with high selectivity and affinity toward target molecules by multivalent binding. Moreover, the sequences of the peptides can be determined by DNA tags. I tried to construct a dipeptide library using three amino acids and succeeded in conjugation of the amino acids and corresponding DNA tags to ssDNA and formation of the triple strand DNA. Selection using this library is now under planning.