Development of versatile method for identification of the target protein using affinity resins with photo-labile linker

我们开发了一种利用裂解产物中的亲和树脂鉴定药物、天然产物等生物活性物质的有效方法。在这种方法中，我们应用光不稳定连接物，将目的蛋白与靶器官的裂解物混合后，从亲和树脂中选择性地洗脱出来。我们使用我们的产品AquaFirmus作为固体材料，因为它的化学稳定性和亲水性足以减少非特异性蛋白质的吸收。我们首先通过带有亲水间隔物的光解连接物将目标已知的生物活性化合物如FK506或苯磺酰胺固定在树脂上，并通过在冰上照射5min成功地洗脱了目标蛋白质。我们现在应用这种新的方法来鉴定目标未知的化合物，包括制药公司的已开发化合物。

We have here develped an effective method for identification of bio-active compound such as drug, natural product, and so on, using affnity resins from lyates. In this method, we applied photo-labile linker to elute the target protein from affinity resins selectively after mixed with lysate from target organ. We used "AquaFirmus", our products, as solid material in this work because this is chemically stable and hydrophilic enough to reduce the non-specific protein absorption.We first fixed target-known bioactive compounds such as FK506 or benzenesulfonamide on the resin through the photo-libile linker with hydrophilic spacers, and successfully eluted the target proteins by irradation on ice for 5 min. We now apply this novel method for identification of target-unknown compounds including developed compounds of pharmaceutical companies.