Metapopulation mapping of endagered freshwater fishes for restoration of the ecosystem in traditional irrigation ponds

佐越山(日本农村地区)的传统灌溉池塘是许多濒危动植物的重要栖息地，其中许多面临着环境变化的危机。尽管许多人经常致力于恢复环境，但大多数栖息地与其他湿地隔绝，因此淡水鱼无法自行从其他栖息地迁徙。为了恢复传统灌溉池塘的动物群，我们必须重新引入其他地区的淡水鱼。在本研究中，我试图重建濒危鱼类伪小亚种的集合种群结构。利用线粒体DNA全序列，比较了同一地区(日本东海地区)其他淡水鱼的系统地理。结果表明，小菜蛾亚种的种群数量较少。该物种在丘陵地区的分布差异很大，并建议应限制在包括源种群在内的小范围内重新引入。

Traditional irrigation ponds in SATOYAMA (rural area of Japan) are important habitats for many endangered animals and plants, and many of them are facing a crisis of environmental changes. Although many people have often been working to restore the environments, most of the habitats are isolated from other wetlands, thus freshwater fishes cannot migrate from other habitats by themselves. To restore the fauna of traditional irrigation ponds, we have to re-introduce the freshwater fishes from the other areas.In this study, I try to reconstruct the meta-population structure of endangered fish, Pseudorasbora pumila subsp. by using complete mitochondrial DNA sequences, and compared the phylogeography of the other freshwater fishes in the same area (Tokai region, Japan). The results indicated that the population of P. pumila subsp. was highly diverged among hilly areas, and suggested that the re-introduction should be restricted in a small landscape including the source population.