Size controlled preparation of functional gold nanoparticles using micelles of reductive-stabilizer

我们开发了一种新的制备金纳米粒子（AuNps）的方法，使用两亲性的烷基氨基苯硫醇和烷基硫烷基苯胺作为还原稳定剂（RS），将Au 3+还原为AuNps，并通过与巯基或氨基配位自发地稳定所得到的AuNps。由于还原反应在RS胶束内进行，因此所得AuNp的形状和稳定性由RS的结构控制。利用这些化合物的混合物，可以制备出不同类型化合物包覆的金纳米粒子。所得到的糖-金纳米颗粒显示出与伴刀豆球蛋白A，一种碳水化合物结合蛋白的特异性和可逆的相互作用。四种具有生物活性的糖模拟物的不对称合成也已完成。我们正计划将它们引入RS，以提供具有生物活性的AuNps。

We have developped a new preparation method of gold nanoparticles (AuNps) using amphipathic alkylaminobenzenthiols and alkylsulfanylanilines as reductive stabilizers (RS) that reduce Au3+ to AuNps and spontaneously stabilize the resulting AuNps by coordination with their thiol or amino group. Since the reduction reaction proceeds inside of the micelles of RS, the shape and stability of the resulting AuNp were controlled by the structure of RS. By using a mixture of these compounds, the AuNps covered with different type of compounds can be prepared.The AuNps covered with sugar molecules were easily prepared with the sugar-derived RS. The resulting sugar-AuNps showed specific and reversible interaction with concanavalin A, a carbohydrate-binding protein. Asymmetric synthesis of four biologically active sugar mimics has also been accomplished. We are planning to introduce them to the RS to give biologically active AuNps.