Discovery of novel enantioselective imine reductases and efficient enzyme synthesis of optically active amines

手性胺是合成药物的有用化合物。在光学活性胺的高效化学合成中，亚胺的不对称还原得到了很好的研究。然而，合成亚胺的酶还原还未见报道。因此，我们对土壤样品中的亚胺还原酶进行了调查，首次发现了R-亚胺还原酶和S-亚胺还原酶。在酶的纯化和鉴定后，进行基因分析和重组细胞的制备。我们利用亚胺还原酶高效地合成了光学纯度较高的旋光性胺类化合物。

Chiral amines are useful compounds for the synthesis of pharmaceuticals. In an efficient chemical synthesis of optically active amine, asymmetric reduction of imine has been well studied. However, enzymatic reduction of synthetic imine have not been reported yet. We therefore surveyed imine reductase from soil samples , and first found R-and S-imine reductases. After purification and characterization of the enzymes, gene analysis and preparation of recombinant cells were performed. We efficiently synthesized optically active amines with high optical purity using their imine reductases.