Elucidation of polarity mechanism in migrating cells and analysis of molecular dynamics of phospholipase D on the cell membrane

细胞迁移在多种正常的生物学过程和癌细胞的转移中起着重要作用。众所周知，细胞迁移是由几个细胞事件控制的（例如，肌动蛋白-细胞骨架、底物粘附、膜交通和细胞极性的调节）。因此，调节细胞迁移的系统非常复杂，需要在迁移过程中对这些细胞事件进行协调的时空控制。到目前为止，我们使用我们的筛选系统筛选了调节细胞迁移的基因，鉴定出PLD和32个激酶相关基因。在这项研究中，我通过可视化PLD及其产物磷脂酸，研究了PLD在细胞膜上的单分子动力学和活化。此外，我对鉴定的基因产物进行了通路分析，构建了一个全面的细胞迁移信号网络。

Cell migration plays a major role in a variety of normal biological processes and metastasis of cancer cells. It is known that cell migration is controlled by several cell events (e.g., regulation of actin-cytoskeleton, substrate adhesion, membrane traffic, and cell polarity). As a consequence, the system regulating cell migration is very complicated, requiring coordinated spatiotemporal control of these cell events during the migration process. Hitherto, we screened genes regulating cell migration by using our screening system, and PLD and 32 kinase-related genes were identified. In this study, I investigated the single molecule dynamics and activation of PLD on cell membrane by visualization of PLD and its products, phosphatidic acid. Furthermore, I performed pathway analysis among identified gene products to construct a comprehensive signal network of cell migration.