Isolation of floral organ morphogenetic genes and elucidation of their involvement in double pistil formation in sweet cherry

本研究分离了甜樱桃花器官形态发生基因，并在不同温度条件和品种下研究了其表达水平与双雌蕊形成和胚珠发育的关系。分离出B类MADS box基因PaTM6和PaPI， C类基因PaAG和PaSHP， D类基因PaSTK，分析其在花各器官中的表达情况。虽然整个实验没有明显的趋势，但我们认为高温导致PaAG基因表达水平升高可能是双雌蕊形成的原因。另一方面，表明PaSTK基因的低表达可能与赤霉素引起的早期胚珠变性有关。

In this study, we isolated the floral organ morphogenetic genes of sweet cherry, and investigated the relationship between their expression level and double pistil formation and ovule development under various temperature conditions and cultivars. The class B MADS box genes, PaTM6 and PaPI, the class C genes, PaAG and PaSHP, and the class D gene, PaSTK, were isolated, and their expression in each flower organ was analyzed. Though there was no clear trend through the whole experiments, it was suggested that the increased expression level of the PaAG gene might be responsible for double pistil formation due to high temperature. On the other hand, it was indicated that the low expression level of the PaSTK gene, might be involved in early ovule degeneration due to gibberellin.