Development of immobilized koji-mold catalyst for effective isolation of pharmaceutically significant yerba mate compounds

将曲霉菌催化剂应用于马黛茶中，建立了一种制备达菲莽草酸等药物原料的新策略。绿原酸（CGA）水解酶（CGase）在尼日尔曲霉等菌丝体中生长时，在马黛茶提取物上被有效地诱导。40 ℃热处理30 min，多酚氧化酶被破坏，而CGase具有很强的抗性，在热处理过程中仍能存活，因此，热处理菌丝体可作为CGase的固定化催化剂。研究了以固定化热菌丝体为底物的几种模式反应。这是本研究中最突出的一点，该方法可用于QA和咖啡酸（CA）的工业生产。QA可以通过乙酸细菌的两种酶系统转化为莽草酸，如在先前的研究中已经建立的。通过简单的离子交换柱色谱法建立了QA、CGA和CA的同时分离。

A novel strategy preparing raw materials for pharmaceuticals such as shikimic acid for Tamiflu was established applying koji-mold catalyst to yerba mate tea. Chlorogenic acid (CGA) hydrolase (CGase) was potently induced in mycelia such as Aspergillus niger when grown on yerba mate extract. Heat treatment of mycelia at 40˚C for 30 min destroyed polyphenol oxidase but CGase was highly resistant and survived during the heat treatment.Thus, the heated mycelia were found useful as a kind of immobilized catalyst of CGase. Some model reaction using immobilized heated mycelia as CGase was studied. This is the most outstanding point in this study and this method can be accepted for industrial production of QA and caffeic acid (CA). QA can be converted to shikimate by two enzyme systems of acetic acid bacteria, as has already been established in the previous study. Simultaneous isolation of QA, CGA, and CA was established by a simple ion exchange column chromatography.