Carbohydrate-recognition and transmembrane ion-channel-formation mechanisms of calcium-dependent lectins

钙依赖凝集素是生物分子识别系统中的重要组成部分，本文分析了钙依赖凝集素的糖识别机制，并设计合成了一种具有特异性结合的新型人工凝集素。此外，溶血凝集素CEL-III的定点突变和三维结构分析显示，它在靶细胞膜上形成具有广泛构象变化的七聚体，形成导致溶血或细胞死亡的跨膜孔。这些结果为研究自然界中广泛分布的致孔毒素蛋白提供了有价值的信息。

Carbohydrate-recognition mechanisms of calcium-dependent lectins, which play important roles in molecular recognition systems in organisms, were analyzed, and a novel artificial lectin with a designed binding-specificity was produced. In addition, site-directed mutational and three-dimensional structural analyses of the hemolytic lectin CEL-III revealed that it forms heptamers in target cell membrane with extensive conformational changes, forming the transmembrane pores that lead to hemolysis or cell death. These results may provide valuable information on various pore-forming toxin proteins widely distributed in the nature.