Synthesis of inner-core oligosaccharides of LPS and its protein conjugates for a definition of the epitope recognized by human antibodies

本研究成功地合成了9种类型的寡糖，它们是致病革兰氏阴性菌产生的脂多糖/脂寡糖的部分内核寡糖。 合成的寡糖用生物素标记，并与人血清白蛋白偶联。 这些偶联物将用于确定人源抗体识别的表位和开发抗致病性革兰氏阴性菌感染的糖疫苗。

In this research, we successively achieved the synthesis of 9 type oligosaccharides, which are partial inner-core oligosaccarides of lipopolysaccharide/lipooligosaccharide produced by pathogenic gram-negative bacteria. And synthesized oligosaccharides were labeled with biotin and also conjugated to human serum albumin. These conjugates will be utilized for the definition of the epitope recognized by human antibody and the development of carbohydrate vaccine against pathogenic gram-negative bacterial infection.