Effects of restrained water immersion stress on the metabolism of histamine in the gastric mucosa.

束缚水浸泡应激30 min（WRS）可引起小鼠和大鼠胃粘膜轻度溃疡。胃粘膜组胺含量分别增加1.6倍和1.3倍。 抗组胺N-甲基转移酶（HMT）抗体在大鼠肾脏中检测到一条带，而在胃粘膜中未检测到。定量PCR检测到胃粘膜中HMT mRNA，但WRS检测结果无变化。 抗组氨酸脱羧酶抗体在大鼠胃粘膜中检测到单一条带，但WRS含量无变化。抗二胺氧化酶抗体未能检测到表达在胃粘膜。 MAP激酶（ERK 1/2、SAPK/KNK、p38）的磷酸化水平不受WRS的影响。

Restrained water immersion stress of 30 min (WRS) in mice and rats caused mild ulcer in the gastric mucosa. Histamine content in the gastric mucosa increased 1.6 and 1.3 times, respectively. Anti-histamine N-methyltransferase (HMT) antibody detected a band in rat kidney, but not in gastric mucosa. Quantitative PCR detected HMT mRNA in gastric mucosa, but the expression level did not change by WRS. Anti-histidine decarboxylase antibody detected a single band in the rat gastric mucosa but the content did not change by WRS. Anti-diamine oxidase antibody failed to detect expression in the gastric mucosa. The phosphorylation level of MAP kinases (ERK1/2, SAPK/KNK, p38) did not change by WRS.