Pain regulation based on quality control of TRPV6, a target molecule of nocistatin-inetarcting protein

我们鉴定了一种痛觉调节多肽Nocistatin相互作用蛋白(Nocistatin Binding Protein，NSP1)。NSP1结合了分子伴侣HSP60和高选择性的钙通道TRPV6。NSP1和HSP60的相互作用在肾脏中观察到，但在中枢神经系统中没有。另一方面，除肾脏外，TRPV6在脑和脊髓中也有表达，TRPV6剪接变异体在中枢神经系统中特异表达。NSP1通过将TRPV6转位到细胞膜来抑制TRPV6的活性。观察炎性疼痛时NSP1和TRPV6剪接变异体表达的变化。

We identified a pain-regulated peptide nocistatin-interacting protein (Nocistatin binding protein, NSP1). NSP1 bound a molecular chaperone HSP60 and a highly selective Ca2+ channel TRPV6. The interaction of NSP1 and HSP60 was observed in the kidney but not the central nervous system. On the other hand,TRPV6 was expressed in the brain and spinal cord in addition to kidney, and TRPV6 splice variants were specifically expressed in the central nervous system. NSP1 inhibited the TRPV6 activity through the translocation of TRPV6 to the cell membrane. The changes of NSP1 and TRPV6 splice variants expression were observed in inflammatory pain.