SEARCHING OF SPECIFIC PROTEINS PRODUCED IN GALLBLADDER CANCER TISSUES FOR THE EARLY DETECTION OF THE CANCER

检测和鉴定胆囊癌早期诊断的特异性蛋白的操作程序如下：从福尔马林固定的石蜡包埋的胆囊癌组织块制备石蜡切片，脱蜡，在显微镜下用Perioic ACID-Schiff染色切片，激光显微切割组织切片，原位直接消化，C18阶段针尖纯化样品，用微量比辛酸蛋白分析测定多肽溶液的浓度，LC/MS/MS分析。6例来自晚期癌细胞(III组)。每组平均检测到的蛋白质数量分别为993、1,291和1,109。在这些蛋白中，群特异性蛋白分别为306、1、148和930。用于胆囊癌早期检测的新生物标志物可能会被发现，以仔细检查这些蛋白。

Our operating procedure to detect and identify the specific proteins as the biomarker for the early detection of gallbladder cancer was as follows: preparation of paraffin sections from formalin-fixed paraffin-embedded gallbladder cancer tissue blocks, deparaffinization, stain sections on a microscope by Periodic acid-Schiff stain, excision of tissue section by laser microdissection, on-site direct digestion, purification of the samples using the C18 Stage Tip, concentration measurement of the peptide solutions by micro bicinchoninic acid protein assay, LC/MS/MS analysis.A total of 15 samples were collected: 3 from normal cell (group I), 6 from cancer cell (group II), and 6 from advanced cancer cell (group III). The average numbers of detected proteins from each group were 993, 1,291, and 1,109. In these proteins, the group specific proteins were 306, 1,148, and 930. The new biomarkers for the early detection of gallbladder cancer may be found to examine these proteins closely.