The role of cell cycle regulator Cdh1 in carcinogenesis and development of gastrointestinal cancer.

用siRNA tequnique抑制结肠癌细胞系Cdh1的表达。这些细胞显示细胞骨架异常，肌动蛋白丝减少。在伤口愈合实验的细胞运动分析中，与对照细胞相比，Cdh1抑制细胞表现出较高的运动性。接下来，我们用tequnique基因诱捕器抑制了Cdh1磷酸化位点之一的异种小鼠。后代基因分型为：野生型：异源型：同源型=13:32:0。这些结果表明，Cdh1磷酸化位点的人属抑制是小鼠胚胎致死事件。现在，我们正在研究死亡时间和死因。

Suppress the expression of Cdh1 with siRNA tequnique in colon cancer cell line. These cells revealed cytoskeletal abnormality with decreased actin filament.In the analysis of cell motility with the wound healing assay, Cdh1 suppresse cells showed high motility compared with the control cells.Next, we generated heterogenous mice which one of the phosphorylation site of Cdh1 was suppressed with gene trap tequnique.Genotyping of offspring of these mice was following as wild type: hetero: homo=13:32:0. These results indicated that homo suppression of Cdh1 phosphorylation site was embryonic lethal event in mice.Now,we are studying about the timing of lethality and cause of death.