iPS cells from diabetic patients

从mtDNAA3243G突变患者(mt-iPS)中成功地获得了iPS细胞，mt-iPS细胞表现出双峰程度的突变异质性。在14个克隆中，有6个的突变频率下降到无法检测到的水平，而其他克隆的突变频率比原始成纤维细胞的突变频率增加了几倍。在连续培养过程中和分化后，未见突变复发或异质性水平发生明显变化。突变丰富、稳定的mt-iPS细胞可能是体外建立人类线粒体疾病模型的合适细胞来源。无突变的iPS细胞可以为自体移植治疗提供无限、无疾病的细胞供应。

iPS cells were successfully generated from patients with the mtDNA A3243G mutation(Mt-iPS).The Mt-iPS cells exhibited a bimodal degree of mutation heteroplasmy. The mutation frequencies decreased to an undetectable level in six of 14 clones, while the others showed several-fold increases in mutation frequencies compared with those in the original fibroblasts. During serial cell culture passage and after differentiation, no recurrence of the mutation or no significant changes in the levels of heteroplasmy were seen. Mutation-rich, stable Mt-iPS cells may be a suitable source of cell for human mitochondrial disease modelling in vitro. Mutation-free iPS cells could provide an unlimited, disease free supply of cells for autologous transplantation therapy.