Identification of cellular genes associated with pathogenesis of chronic active Epstein-Barr virus infection

慢性活动性EB病毒感染(CAEBV)的发病机制尚不清楚。以前的研究已经确定了一些与CAEBV相关的候选细胞基因。然而，关于CAEBV患者新鲜分离细胞中基因表达水平的数据很少。在本研究中，采集了CAEBV患者的外周血样本。我们研究了CAEBV中宿主细胞的基因表达谱，使用了一种专注于T细胞和B细胞激活的PCR阵列分析。基于PCR芯片分析和随后的实时定量PCR分析，我们发现CAEBV患者中有四个基因(IL-10、IL-2、IFNGR1和INHBA)上调，这四个基因与对照组相比表现出10倍的差异。这些基因可能与炎症反应和细胞增殖有关，可能与CAEBV的发生发展有关。

The pathogenesis of chronic active Epstein-Barr virus infection (CAEBV) remains unclear. Previous studies have determined a number of candidate cellular genes associated with CAEBV. However, few data are available on the gene expression levels in freshly isolated cells from patients with CAEBV. In the present study, peripheral blood samples were obtained from patients with CAEBV. We investigated the host cellular gene expression profiles in CAEBV using a PCR array analysis that focused on T-cell and B-cell activation. We identified that four genes (IL-10, IL-2, IFNGR1, and INHBA) were upregulated in the CAEBV patients based on the PCR array analysis and the subsequent quantitative real-time PCR assay, where these four genes exhibited tenfold differences compared with the control subjects. These genes may be associated with inflammatory responses and with cell proliferation, and they may contribute to the development and progression of CAEBV.