Early differential diagnosis of malignant melanoma by abnormal DNA methylation

为了确定恶性黑色素瘤的早期肿瘤标志物，采用Massarray Compact系统进行Sequenom Massarray定量甲基化分析，对ZAR1基因进行定量DNA甲基化分析。对病理诊断与恶性黑色素瘤相似的第五例手术标本(如黑色素细胞痣、Spitz痣、发育不良痣和原位黑色素瘤)进行检查。用徕卡激光显微切割系统采集DNA。然而，使用MassARRAY进行ZAR1基因的DNA提取和DNA甲基化定量分析是困难的，因此我们无法进行分析。

In order to identify the early tumor marker of malignant melanoma, Sequenom MassARRAY quantitative methylation analysis using the MassARRAY Compact System was performed for the quantitative DNA methylation analysis of ZAR1 gene. Fifth surgical specimens (e.g. melanocytic nevus, Spitz nevus, dysplastic nevus, and melanoma in situ) that were similar to malignant melanoma by pathologic diagnosis were examined. DNA were collected by using Leica Laser Microdissection systems. However, the extraction of DNA and the quantitative DNA methylation analysis of ZAR1 gene using the MassARRAY were difficult, therefore we were not able to analyze.