Analysis of amino acid-signal transduction in the intestinal system, applying a new nipple-bottle feeding system and artificial milk formula for newborn mice.

应用一种新的小鼠乳头瓶喂养系统，用谷氨酰胺缺失的人工奶粉喂养新生小鼠，导致新生小鼠因上皮细胞死亡而导致结肠出血的发生率较高。低浓度谷氨酰胺培养的IEC6大鼠肠上皮细胞发生凋亡，谷氨酰胺缺乏可能通过细胞凋亡导致肠上皮细胞排列不稳定。

We have presented that feeding neonatal mice with glutamine-deleted artificial milk formula induced high incidence of colonic hemorrhage due to the epithelial cell death, applying a new nipple-bottle feeding system for newborn mice. IEC6 rat intestinal epithelial cells cultured with low concentration of glutamine underwent apoptosis.Glutamine deprivation might cause instability of the intestinal epithelial alignment by apoptosis.