Identification of pancreatic stellate cell associated-microRNA that suppress tumor-stromal interaction and clarification of its mechanism.

我们分析了来自胰腺癌和正常胰腺的胰腺星状细胞(PSCs)的microRNAs。基因芯片显示，与正常PSCs相比，癌相关PSCs中miR-31和miR-34a表达增加，miR-21表达降低。接下来，我们建立了高转移性胰腺癌细胞，以确定与转移相关的MIR。在高转移的Suit-2细胞(MSCs)中，基因芯片显示与亲本细胞相比，MSCs中miR-125b-5p、miR-4324和miR-4706表达增加，miR-16-5p、miR-21-5p、miR-192、miR-194和miR-5100表达降低。与亲本细胞相比，高转移PANC-1细胞miR-1247-3p、miR-2964a-5p和miR-3135b表达增加，miR-377-5p、miR-4454和miR-5100表达降低。我们已经从胰腺星状细胞、高转移胰腺癌细胞和IPMN中鉴定出几个miRNAs，它们有可能成为胰腺癌预后价值的标志物。

We analyzed microRNAs of pancreatic stellate cells(PSCs) derived from pancreatic cancer and normal pancreas. Microarray showed miR-31 and miR-34a increased in cancer associated PSCs and miR-21 decreased compared with normal PSCs. Next, we established highly metastatic pancreatic cancer cells to identify the miRs that associate with metastasis. In highly metastatic SUIT-2 cells(MSCs), microarray showed that miR-125b-5p, miR-4324 and miR-4706 increased and miR-16-5p, miR-21-5p, miR-192, miR-194 and miR-5100 decreased in MSCs compared with parent cells . In highly metastatic PANC-1 cells(MPCs), miR-1247-3p, miR-2964a-5p and miR-3135b increased and miR-377-5p, miR-4454 and miR-5100 decreased in MPCs compared with parent cells. We have identified several miRNAs from pancreatic stellate cell, highly metastatic pancreatic cancer cells and IPMN that has possibility of becoming markers for prognostic value of pancreatic cancer.