The role of Necl-5 in the invasive activity of lung adenocarcinoma

在这项研究中，我们使用了一种易于制备的双层胶原凝胶半球(DL-CGH)系统，利用该系统可以可视化细胞在侵袭过程中的迁移，利用该系统，我们使用细支气管肺泡癌(BAC)细胞株和成纤维细胞来评估NECL-5在肿瘤-间质沟通发展中的表达。Necl-5在BAC细胞中的表达高于成纤维细胞。即使当BAC细胞与成纤维细胞混合时，这一趋势也不会改变。为了评估Necl-5在BAC细胞侵袭活性中的作用，我们使用RNA干扰(RNAi)下调了它的表达。用DL-CGH进行侵袭实验表明，Necl-5在BAC细胞中的表达受抑制与侵袭力受抑制有关。肺癌细胞中Necl-5的表达是肿瘤-间质相互作用中侵袭性的关键，提示Necl-5可能是抑制肺腺癌侵袭性的一个有利的分子靶点。

In this study,we used a bronchioloalveolar carcinoma (BAC) cell line and fibroblasts to assess the expression of Necl-5 in the development of cancer–stroma communication by using an easy-to-prepare double-layered collagen gel hemisphere (DL-CGH) systemthat enables visualization of cellmigration during invasion. The expression of Necl-5 was higher in BAC cells than in fibroblasts. This tendency didn't change even when the BAC cells were mixed with fibroblasts. To assess the role of Necl-5 in the invasive activity of the BAC cells, we knocked down its expression using RNA interference (RNAi). The invasion assay with DL-CGH revealed that inhibition of Necl-5 expression in the BAC cells was associated with suppressed invasiveness. Necl-5 expression in lung cancer cells is crucial for their invasiveness in the cancer–stromal interaction, suggesting that Necl-5 could be a favorable molecular target for the suppression of invasiveness in lung adenocarcinoma.