Mechanism of glioma invasion and its inhibition

用视频监视器观察培养的胶质瘤细胞的运动情况。胶质瘤细胞系A172在其他细胞如U87、T98G和原发性胶质瘤细胞培养中是最活跃的细胞。低氧舱和低pH刺激下的细胞表现为细胞初始停滞，随后呈高运动性。这些运动的抑制作用被干扰素阻断。对于侵袭前沿的检测，近年来在胶质瘤手术中引入了5ALA光动力学诊断。用培养细胞测定荧光强度和细胞密度。报告了操作人员肉眼检测限。

The motility of cultured glioma cells were estimated by video monitor. The glioma cell line A172 was the most moving cells in other cells such as U87, T98G and primary glioma cell culture. The cells stimulated by low Oxygen chamber and low pH showed initial cell halt and following high motility. The inhibition of these motility was blocked by interferon beta. As for the detection of invasion front, recently 5ALA photodynamic diagnosis was introduced in glioma surgery. Using cultured cells, the fluorescent intensity and cell density were determined. The detection limit by the operator's naked eyes was reported.