elucidation of the molecular mechanisms about the protective effect of hydrogen against renal ischemia-reperfusion injury

活体器官缺血再灌注损伤，极大地改变了生命预后。研究的重点是氢作为一种具有器官保护作用的药物的肾保护作用。采用Wistar大鼠，麻醉强制，使气管插管，人工呼吸，后暴露于肾脏两侧腹部切口，对肾动脉、肾静脉、输尿管进行整体夹持。缺血时间为40分钟。按低浓度组和高浓度组给氢。之后，我缝合了伤口。24h后采血，测定血尿素氮、肌酐。经给氢，血尿素氮升高，肌酐可被抑制。

Organ ischemia-reperfusion injury in a living body, greatly changed the life prognosis. The studies were conducted with a focus on the renal protective effect of hydrogen as an agent with the organ protective effects. Use the Wistar rat, anesthesia enforcement, make tracheal intubation, artificial respiration, after exposure to the kidney on both sides side abdominal incision, to clamp en bloc renal artery, renal vein, ureter. Ischemic time is 40 minutes. Administration of hydrogen was performed by dividing the low concentration and high concentration group. After that, I closed the wound. Blood samples were collected after 24 hours, and measured blood urea nitrogen, and creatinine. By hydrogen administration, increased blood urea nitrogen, creatinine can be suppressed has been found.