Basic research of smooth muscle cells generated from induced pluripotent stem cells for urethral tissue engineering.

压力性尿失禁与生活质量下降有关。完整的尿路括约肌是维持尿控的必备条件。成人干细胞注射治疗受损括约肌的再生修复目前处于研究的前沿。最近，iPS细胞的建立为产生患者特异性干细胞系提供了一种新的治疗策略。在这里，我们研究了iPS细胞是否能够在体外分化为平滑肌细胞(SMCs)。用10-5mol/L全反式维甲酸(RA)处理人iPS细胞单层。经RA处理7天后，免疫荧光法检测到iPS细胞高表达SMC标志物，包括SMA-肌动蛋白、SM肌球蛋白HC、myocardin和结蛋白。本研究建立了一种简单的iPS-SMC体外培养系统，为组织工程和尿失禁修复提供个性化的SMC，具有巨大的潜力。

Stress urinary incontinence is associated with a reduced quality of life. An intact urethral sphincter is mandatory to maintain urinary continence. Adult stem cell injection therapy for the regenerative repair of an impaired sphincter is currently at the forefront of research. Recently, the establishment of iPS cells has offered a novel therapeutic strategy to generate patient-specific stem cell lines. Here we have investigated whether iPS cells are able to differentiate into smooth muscle cells (SMCs) in vitro. Human iPS cell monolayers were treated with 10-5 mol/L all-trans retinoid acid (RA). After 7 days of RA treatment, we found that iPS cells highly expressed the SMC-markers including SMa-actin, SM myosin HC, myocardin, and desmin determined by immunofluorescence. Our date have established a simple iPS-SMC system to generate SMCs in vitro, which has tremendous potential to generate individualized SMCs for urethral tissue engineering and continence recover.