Study of the alteration with aging of human oocyte spindle function using extracted human oocyte in operation.

本研究采用8例I期子宫内膜癌患者的卵巢标本。通过穿刺卵巢卵泡获得未成熟卵母细胞。30岁组（a组）获得的卵母细胞数量明显多于40岁组（B组）。在IVM培养基中培养后，我们从A组的未成熟卵母细胞中获得成熟卵母细胞，而从b组的未成熟卵母细胞中获得成熟卵母细胞，这表明衰老影响卵母细胞的成熟。免疫荧光染色观察到纺锤体排列不规则，但也观察到纺锤体排列规则。微管信号强烈地定位于细胞膜附近。这表明微管与细胞膜之间存在相互作用。在A、b两组中，周心蛋白的信号均与纺锤体和细胞膜接触，并无差异。由此，我们建立了从手术中取出的卵巢中获取可用卵母细胞的方法。

It was used extracted ovaries from eight patients of endometrial carcinoma stage I for this study. We obtained immature oocytes by the puncture on the follicles in ovaries. The number of obtained oocyte of a group of thirties (group A) was more than that of a group of forties (group B) significantly. After the culture in IVM medium, we obtained mature oocytes from immature oocytes of group A, but not from those of group B. It suggests the aging affects the oocyte maturation. We observed irregular arrangement of spindle but also regular one in immunofluorescence staining. The signal of microtubule localized near cell membrane intensely. It suggested the interaction between the microtubule and the cell membrane. The signal of pericentrin existed in contact with spindle and cell membrane and were not different between group A and B.From the above, we established the method that we obtained the available oocytes for investigations from extracted ovaries in operations.