Research on the regeneration of tracheal cartilage using adipose-derived stem cell

本研究的目的是使用由分化的脂肪干细胞(ADSC)制成的软骨细胞片来构建圆柱形软骨。从白兔皮下脂肪组织中获取ADSCs，并进行培养，以促进软骨细胞片的形成。虽然ADSC向软骨细胞的分化最初并不令人满意，但通过添加多种生长因子和调整培养条件，最终形成了软骨细胞片。然而，由于缺乏丰富的软骨基质，通过旋转培养软骨细胞片来构建圆柱形软骨是困难的。结论：使用ADSCs来源的软骨细胞构建无支架的圆柱形软骨是非常困难的，足够的支架，如细胞外基质，对于干细胞再生气管软骨是必不可少的。

The aim of this study was to fabricate a cylindrical cartilage using cartilaginous cell sheets that were made by differentiated adipose-derived stem cells (ADSC). ADSCs were harvested from the subcutaneous adipose tissue of white rabbits and cultured in order to promote a formation of cartilaginous cell sheet. Although a differentiation from the ADSC to the cartilaginous cell was initially unsatisfactory, cartilaginous cell sheets were finally formed by the addition of several growth factors and the adjustment of culture conditions. However it was difficult to fabricate a cylindrical cartilage by a rotative incubation of cartilaginous cell sheets due to a lack of abundant cartilage matrix. It was concluded that a fabrication of a scaffold-free cylindrical cartilage using cartilaginous cells originated from the ADSCs was extremely difficult and adequate scaffold, such as extracellular matrix, was essential to a regeneration of tracheal cartilage from the stem cells.