Development of a method to remove mycoplasmas from the contaminated cell cultures using antimicrobial photodynamic therapy

支原体是细胞培养中最常见的污染物之一。需要开发一种在不使用抗生素的情况下清除支原体的方法，因为使用抗生素治疗可能会产生抗药性细菌。这项研究试图通过使用无绳式红色发光二极管的抗菌光动力疗法来去除细胞培养中污染唾液支原体(MS)。将感染MS的人胚胎肾293细胞用红光二极管照射，以亚甲基蓝为光敏剂，用无菌磷酸盐缓冲液冲洗减少活的MS数。经抗菌光动力治疗后，支原体检测试剂盒和培养的细胞中均未检测到MS，表明抗菌光动力学治疗成功地将MS从其污染的细胞中去除，而不会对细胞造成损害。

Mycoplasmas are one of the most frequent contaminants due to their characteristics in cell cultures. It is required to develop a method to remove mycoplasmas without using antibiotics, because a treatment with antibiotics may induce antibiotic-resistant bacteria. This study attempted to remove cell culture-contaminating Mycoplasma salivarium (Ms) by utilizing antimicrobial photodynamic therapy with a cordless red light emitting diode. Ms-infected human embryonic kidney 293 cells was irradiated with red light emitting diode in the presence of methylene blue as a photosensitizer, after decreasing the number of live Ms by washing with sterilized phosphate-buffered saline. No MS was detected in the contaminated cells by both mycoplasma detection kit and cultivation after antimicrobial photodynamic therapy, demonstrating that antimicrobial photodynamic therapy successfully remove Ms from its contaminated cells without giving damages to the cells.