A new induction method for the controlled differentiation of human iPS cells using frozen sections

iPSC本身也存在一些需要克服的问题。 简而言之，一种简单而有效的方法已经被期望用于诱导iPSCs的分化和质量评估。 在这项研究中，我们假设有可能通过在具有最适合诱导的微环境的每个组织/器官的冷冻切片上培养来诱导iPSCs的受控分化。 结果，在肝脏上培养的iPSC表达肝细胞相关标志物的mRNA和蛋白质，并且在脑/脊髓上培养的iPSC分别表达神经元相关标志物的mRNA和蛋白质。 此外，iPSC在冷冻切片上的分化效率在iPSC的克隆之间可能不同。 从这些事实判断，使用冷冻切片的人iPSC的诱导方法可以用作诱导iPSC分化和评估iPSC的潜力和/或质量的简单有效的措施。

iPSCs themselves have some problems that need to be overcome. Briefly, a simple and efficient method has been expected for the induction the differentiation and the evaluation of quality of iPSCs. In this study, we hypothesized that it is possible to induce the controlled differentiation of iPSCs by culturing on frozen sections of every tissue/organ which have the most suitable microenvironment for the induction. As a result, iPSCs which were cultured on liver expressed mRNA and protein for hepatocyte related markers, and iPSCs which were cultured on brain/spinal cord expressed mRNA and protein for neuron related markers, respectively. Moreover, the differentiation efficiency of iPSCs on frozen sections could be different among clones of iPSCs. Judging from these facts, the induction method of human iPSCs using frozen sections could be useful as a simple and effective measure for inducing the differentiation of iPSCs and evaluating the potential and/or quality of iPSCs.