A study on optimum orthodontic force using periodontal ligament fibroblasts on 3D culture system with aging

牙周膜（PDL）成纤维细胞的生理功能减退被认为是随着年龄的增长而进展。在本研究中，尝试根据在3D培养系统上的PDL成纤维细胞在每次压缩负荷后与破骨细胞分化相关的细胞因子的表达随老化的变化来确定最佳正畸力。从这些结果来看，虽然没有观察到作为老化指标的8-OHdG的差异，细胞因子mRNA表达的相对量与其他压缩时间和1/4压缩时间之间的表达趋势相似，但在1/4压缩时间存在更高的趋势。有人建议，当机械压缩被添加到PDL成纤维细胞在一个实验系统，假设在体内，我们认为这是有效的牙齿移动正畸治疗时，牙齿被推的距离的PDL的宽度的四分之一。

The physiological hypofunction of periodontal ligament (PDL) fibroblasts were considered to be progressing with age. In this investigation, an attempt was made to determine the optimal orthodontic force according to the change of expression of cytokines related to the osteoclasts differentiation after each compressive load on PDL fibroblasts on 3D culture system with aging.From these results, though the difference of 8-OHdG which is an indicator of aging was not observed,the relative amounts of the mRNA expression of cytokines were similar to the expression tendencies between the time of other compressions and the time of 1/4 compression, but there was a higher tendency at the time of 1/4 compression. It has been suggested that when mechanical compression is added to PDL fibroblasts in an experimental system that is assumed as in vivo, and we believe it is effective to move teeth in orthodontic treatment when the tooth was pushed by a distance of a quarter of the width of PDL.