Effects of early life stress on transcription or translation of developmental brain nerve cells

包括自闭症在内的精神障碍的发病机制尚不清楚。本研究的目的是阐明早期应激暴露与大脑表观遗传基因调控之间的关系。探讨这些表观遗传基因调控对大脑发育的影响可能性也是研究的另一个目的。有一些报道说，早期生活事件可以引起生理和行为的持久变化。我们选择母婴分离作为早期生活压力。这种分离从出生后的第一天开始，持续一到两周。测量了血液中的皮质酮。两组间无显著差异。在此之后，我们开始了第二次分离，并在出生后第7天制备了中脑总RNA样本。利用DNA甲基化和微阵列技术探索新的靶基因。我们没有得到独特的靶基因。

It is not clear about mechanism of pathogenic mechanism of mental disorder including autism. The purpose of this study is to elucidate the relation between early stress exposure and epigenetic gene regulations in the brain. It is also another purpose to explore the possibility that these epigenetic gene regulations exert influence to growth of brain development.There are some reports that early life events can induce long lasting changes in physiology and behavior.We chose Mother infant separation as early life stress.This separation starts from one day after birth for one week or two weeks.Corticosteron in the blood was measured. There was no significant difference between two groups.After this, we started another separation and total RNA samples of mesencephalon were prepared in seventh day after birth.New target genes were explored with DNA methylation and micro array. We did not get unique target genes.