Development of molecular electrode

在这项研究中，我们检测了pH响应性化合物的细胞毒性和危险剂量，发现当浓度低于几mM时，这些分子是低毒的。我们还制作了一种灵敏的检测装置，可以对具有非常宽的ESR线宽和低S/N比的化合物进行精密的分析。利用X-波段ESR波谱或Overhaser增强磁共振成像，在0.1个分辨率下分析了pH值在6到9之间的变化。我们还合成了五个对pH变化敏感的新分子，并对化合物的物理性质进行了研究。基于这些结果，我们能够在体外和体内测量潜在的变化。由于需要将分子传递到感兴趣的区域，因此通过改进分子设计，例如增加组织和细胞器的传递特性，可以进一步提高本方法的有效性。

In this study, we examined cytotoxicity and hazardous dose of pH responsive compounds and found these molecules are low toxic when the concentrations are lower than several mM. We also made a sensitive detecting device which allows a sophisticated analysis of the compounds which has very wide ESR linewidth and low S/N ration.With the use of X-band ESR spectroscopy or Overhauser enhanced MRI, the pH changes between 6 to 9 was analyzed in 0.1 resolutions. We also synthesized five new molecules that are sensitive to pH changes, and physical properties of the compounds were investigated. Based on the results, we are able to measure potential changes both in vitro and in vivo. Since it is necessary to deliver the molecule to region of interest, the effectiveness of the present method can be improved further by advancing the molecular design, such as increasing the tissues and organelles delivery properties.