Effects of serum albumin binding molecules on embryo culture and analytical system development for the molecules

为了评估非特异性或特异性结合人血清白蛋白（HSA）的小分子，包括市售样品和人血清，我们评估了从人血清中纯化HSA前后的小分子。检测胆红素和胆固醇产生的信号。我们发现使用乙醇的纯化方法是必要的，以去除胆红素。在培养基中加入纯化的HSA后，2细胞小鼠胚胎发育为囊胚。将小鼠胚胎置于添加荧光标记牛血清白蛋白（BSA）的培养基中，观察小鼠囊胚中BSA的荧光。基于这些结果，我们认为在小鼠胚胎发育过程中，受精卵细胞可以摄取HSA。即使有机分子与HSA结合，也能观察到小鼠的胚胎发育。由于不加入影响人胚胎培养的盐和乙醇，很难去除胆红素和胆固醇等结合分子，因此我们得出结论，从人血清中纯化的HSA不能用于胚胎培养。

To evaluate small molecules non-specifically or specifically bound to the human serum albumin (HSA) including commercially available samples and human serum, we evaluated the small molecules before and after purification of HSA from human serum. The signals derived from bilirubin and cholesterol were detected. We found that a purification method using ethanol was necessary in order to remove bilirubin. 2-cell mouse embryo developed to blastocyst when purified HSA was added to the medium. Furthermore, when the mouse embryo is cultured in culture medium supplemented with fluorescence labeled bovine serum albumin (BSA), fluorescence of the BSA was observed in mouse blastocyst. Based on these results, we considered that fertilized oocyte could uptake the HSA in mouse embryonic development. Even if organic molecules bound to HSA, mouse embryonic development was observed. Because removal of binding molecules such as bilirubin and cholesterol is difficult without addition of salt and ethanol that affect the human embryo culture, we concluded that the HSA purified from human serum was not able to use for the embryo culture.