Development of activation technology for cell-free system under microwave irradiation

蛋白质表达的新技术对于蛋白质晶体结构、生物材料和医学都是必要的。无细胞蛋白表达系统有望引领未来的生物工程。为了达到加速酶促反应活性的目的，我们研制了一种微升反应标度的微波涂布器。本研究利用谐振腔型微波应用器对GFP蛋白的表达和DNA聚合进行了研究。结果证实了微波效应对酶促反应的影响。

New technology of protein expression is necessary for protein crystal structure, biomaterial and medical science. The cell free protein expression system is expected to lead the future bioengineering. To achieve acceleration of activity of enzymatic reactions, we prepared an original microwave applicator for microliter reaction scale. In this study, expression of GFP protein and DNA polymerization were demonstrated by using resonant cavity type of microwave applicator. As a result, we confirmed the microwave effectto enzymatic reactions.