Amelioration of Acute Tubular Necrosis in Ischemic Acute Renal Failure was Impaired in Mice Lucking Hypoxia Inducible Factor-1α Gene

用HIF-1αkd小鼠和野生型小鼠IRI模型的肾脏标本进行基因芯片，经过详尽的基因表达分析，发现了一些被认为与应激反应、免疫反应和细胞组织修复相关的基因。我们关注了其中的“CHAC1”基因，并进行了分析。通过HK-2细胞的体外验证实验，可以证实CHAC1基因的表达在实时定量聚合酶链式反应中显著上升，并且能够在蛋白质印迹中证实类似的蛋白水平的表达。基于这些结果，芯片实验证实了缺氧诱导因子-1α对CHAC1的诱导作用，但目前仍在进行中。

As a result of DNA microarrays were performed using kidney sample of the IRI model of HIF-1αkd mice and WILD type mice, and having analyzed exhaustive gene expression analysis, some genes which were thought to be associated with a stress reply and an immune response, cellular tissue repair were found to be present. We paid its attention to "CHAC1" gene of those and pushed forward an analysis. By the in vitro confirmation experiment using HK-2 cells, it could be confirmed that the expression of CHAC1 gene rose in real time PCR conspicuously and was able to confirm similar expression ata protein level in Western blot. Based on these results, the inductivity by HIF-1α of CHAC1 is confirmed using CHIP assay, but is progressing now.