Immunological responses against human papilloma virus and human papilloma virus induced laryngeal cancer

目的：本研究旨在阐明人乳头瘤病毒感染或癌变时喉部的局部免疫状态。方法：对31例喉部疾病患者进行喉部显微手术时的细胞学标本（用于人乳头瘤病毒检测）和喉部分泌物（用于免疫球蛋白评估）。组织学检查有鳞癌12例，喉乳头状瘤4例，其他良性喉部疾病15例。细胞学样本采用杂交捕获2法检测人乳头瘤病毒DNA。结果：12例喉癌患者中有3例检测到高危人乳头瘤病毒DNA，占25%。低危性人乳头瘤病毒DNA仅在3例喉乳头瘤患者中检测到。人乳头瘤病毒DNA阳性患者喉部分泌免疫球蛋白M、G、A和分泌免疫球蛋白A的平均浓度均大于1，是人乳头瘤病毒DNA阴性患者的2倍。两组患者分泌性免疫球蛋白A浓度差异有统计学意义。喉癌患者喉部各免疫球蛋白类型的分泌浓度均高于喉癌良性病变患者。本研究评估了12例喉癌患者喉部各免疫球蛋白类型的平均分泌浓度，比较了人乳头瘤病毒DNA阳性患者（n = 3）和人乳头瘤病毒DNA阴性患者（n = 9）；人乳头瘤病毒DNA阳性肿瘤患者免疫球蛋白M、G、A和分泌性免疫球蛋白A的平均浓度趋于高于人乳头瘤病毒DNA阴性肿瘤患者。结论：人乳头瘤病毒感染或致癌性激活了喉部局部免疫应答。研究结果强烈提示，分泌IgA对喉部与人乳头瘤病毒相关的感染或癌变具有抑制活性。少

Objective: This study aimed to clarify the local immune status in the larynx in the presence of infection or carcinogenesis associated with human papilloma virus. Methods: Cytological samples (for human papilloma virus detection) and laryngeal secretions (for immunoglobulin assessment) were obtained from 31 patients with laryngeal disease, during microscopic laryngeal surgery. On histological examination, 12 patients had squamous cell carcinoma, four had laryngeal papilloma and 15 had other benign laryngeal disease. Cytological samples were tested for human papilloma virus DNA using the Hybrid Capture 2 assay. Results: High risk human papilloma virus DNA was detected in 25 per cent of patients (three of 12) with laryngeal cancer. Low risk human papilloma virus DNA was detected only in three laryngeal papilloma patients. The mean laryngeal secretion concentrations of immunoglobulins M, G and A and secretory immunoglobulin A in human papilloma virus DNA positive patients were more than t … More wice those in human papilloma virus DNAnegative patients. A statistically significant difference was observed between the secretory immunoglobulin A concentrations in the two groups. Patients with laryngeal cancer had higher laryngeal secretion concentrations of each immunoglobulin type, compared with patients with benign laryngeal disease. The study assessed the mean laryngeal secretion concentrations of each immunoglobulin type in the 12 laryngeal cancer patients, comparing human papilloma virus DNA positive patients (n = 3) and human papilloma virus DNA negative patients (n = 9); the mean concentrations of immunoglobulins M, G and A and secretory immunoglobulin A tended to be greater in human papilloma virus DNA positive cancer patients, compared with human papilloma virus DNA negative cancer patients. Conclusion: These results suggest that the local laryngeal immune response is activated by infection or carcinogenesis due to human papilloma virus. The findings strongly suggest that secretory IgA has inhibitory activity against infection or carcinogenesis associated with human papilloma virus in thelarynx. Less