Validity of blood tests and use of genetic profiles for detection of unknown diagnostic items

我们在严重脓毒症患者治疗前后进行了基因分析。评价了获得的结果，并比较了那些从脓毒性休克中恢复的严重脓毒症治疗，如内毒素吸附治疗（多粘菌素-B直接血液灌流）和那些长期休克而对治疗无反应的患者。无论休克状态是否改善，重症监护下10种miRNA增加，8种减少。其中，在治疗后血压升高的病例中有7种类型的miRNA。针对这7种类型，我们分析了明确血压升高患者中发生变化的miRNA的靶基因，确认了94种与血压升高相关的血液相关基因。目前，我们正在分析mRNA和miRNA的通路。

We carried out gene profiling before and after treatment in patients with severe sepsis. The results obtained were evaluated and compared between those who recovered from septic shock with treatments for severe sepsis, such as endotoxin adsorption therapy (polymyxin-B direct hemoperfusion) and those who were in prolonged shock without responding to treatment. Regardless of shock state improvement, 10 types of miRNA increased, and 8 types decreased, with intensive care. Among them, there were 7 types of miRNA in cases with blood pressure elevation in response to treatment. Regarding these 7 types, we analyzed the target genes of miRNA that was changed in patients with definitely elevated blood pressure.We confirmed 94 types of blood-related genes contributing to blood pressure elevation. At present, we are analyzing the pathways of mRNA and miRNA.