The effect of glycosyltransferase GnT-V in the development and progression of oral cancer and evaluation by PET.

细胞表面糖链的形态和表达在癌变过程中发生了很大的变化，在肿瘤的发展中起着重要的作用。为了阐明糖基转移酶GnT-V在癌症发展中的作用，我们通过PET扫描检查了GnT-V在口腔癌中的表达。与正常口腔黏膜组织相比，口腔癌组织中GnT-V mRNA和蛋白表达均明显增强。FDG-PET和FMISO-PET缺氧显像高SUV组，GnT-V和HIF-1α有较高表达的趋势。缺氧条件下GnT-V的表达和功能增加。用n -聚糖加工抑制剂苦马豆素处理人口腔鳞癌细胞株SAS后，细胞活力明显降低。这些结果表明，GnT-V的糖基化与口腔癌细胞的运动和侵袭增强有关，并与口腔癌的进展有关。

The pattern and expression of cell surface sugar chains greatly alter during the carcinogenesis and play important role in tumor progression. To clarify the role of glycosyltrasferase GnT-V in cancer development we examined the expression of GnT-V in the oral cancer association with the PET scan. Both GnT-V mRNA and protein are strongly enhanced in oral cancer compared with normal oral mucosa tissue. In the group with high SUV on FDG-PET and FMISO-PET hypoxia imaging, GnT-V and HIF-1α had tendency to express higher. The expression and function of GnT-V may increase in hypoxia condition. By treatment the human oral squamous cell carcinoma cell line SAS with swainsonine, an inhibitor of N-glycan processing, cell motility was reduced significantly. These results showed that the glycosylation by GnT-V is associate with cell motility and invasion enhancement in oral cancer and related in oral cancer progression.