The 11q13.3 amplicon and mechanisms of bone invasion in in oral cancer

以FADD为中心的11q13.3结构域基因的功能分析，通过在源自口腔癌的培养细胞中使用遗传修饰的突变分子强制表达系统阐明癌症爆发机制，并且还通过对基因组结构异常的综合分析，目的是还检查预测不仅在FADD中而且在11q13.3中具有新重要性的基因。domain基因[The 11q13.3结构域基因对强制表达系统生死的影响]我们克隆了11q13.3结构域基因之一FADD的全长cDNA，并将其亚克隆到表达载体上。但是这些载体中的蛋白表达非常弱，不能通过Western blotting证实，因此我们引入腺病毒并再次进行亚克隆.目前，病毒载体的亚克隆已经停止，我们正在确认表达。[Gene使用微阵列数据的11q13.3结构域的分析]我们通过从口腔癌切除标本的福尔马林固定石蜡包埋块中提取DNA并进行CGH微阵列分析来确认11q13.3结构域的扩增。通过增加CGH微阵列分析的例数，缩小了11q13.3区域内的详细扩增范围，确定了11q13.3区域内癌症的发生和参与该特性的候选基因.

With the functional analysis of 11q13.3 domain gene centered around FADD, by clarifying the cancer outbreak mechanism using genetically modified mutational molecular forced expression system in the cultured cell derived from oral cancer and also by a comprehensive analysis of the genome structure abnormality the aim was to examine also the gene which was predicted to be of new importance in not only the FADD but in the11q13.3 domain gene.【The influence on the life and death of the forced expression system in the 11q13.3 domain gene】We cloned the full length cDNA of FADD which is one of the 11q13.3 domain gene and sub cloned it to the expression vector. However the protein expression in these vectors was very weak that it could not be confirmed by western blotting so we introduced an adenovirusand carried out sub cloning again. At present the sub cloning to the virus vector has ceased and we are up to confirming the expression.【Gene analysis of 11q13.3 domain using the microarray data】We are confirming the amplification of the 11q13.3 domain by extracting DNA from a formalin fixation paraffin embedded block of the oral cancer excision specimen and carried out a CGH microarray analysis. By increasing the case of CGH microarray analysis the detailed amplification range within the 11q13.3 domain is narrowed down and theoccurrence of cancer and the gene candidate which participated in the property in the11q13.3 domain was identified.