Development of the novel principles of electrochemical sensors using zymogens as sensing elements

我们已经开发了新型的电化学传感器使用的酶原作为传感器元件。首先，我们以含酶原的鲎试剂为基础，制备了电化学内毒素传感器。我们成功地开发了一种具有实用灵敏度的成本效益的丝网印刷传感器芯片。我们还开发了一种具有实用结构的高灵敏度溶出伏安法装置。然后，我们将这种基于酶原的传感原理扩展到其他检测中。我们成功地用电化学方法检测了HepG2细胞的凋亡。这些结果不仅有助于传感器工程的发展，而且有助于临床安全性和生物学研究。

We have developed novel type electrochemical sensors using zymogens as sensor elements. First, we made electrochemical endotoxin sensors based on the Limulus amebocyte lysate reagent containing zymogens. We successfully developed a cost effective screen-printed sensor chip with practical sensitivity. We also developed a highly sensitive device with a practical structure for stripping voltammetry. Then, we extended this zymogen-based sensing principle into other assays. We successfully detected the apoptosis of HepG2 cells using electrochemical methods. These results contribute not only the development of the sensor engineering but also the clinical safety and biological studies.