Identifying the unknown novel fungal groups frequently detected in deep-sea and subsurface environments and clarifying their ecological role

本研究从甲烷冷渗漏、10000米深海沟和鲸鱼坠落点等独特的深海环境中分离出300多种真菌。此外，利用单细胞基因组学和下一代测序仪对深海环境中的真菌多样性进行分析，发现了许多新的未知真菌类群。提示甲烷冷渗中频繁检出的类群是高度新颖的早分化类群。本研究通过分离许多新的和未知的物种，检测未知的真菌类群，以及对深海环境中仅报道的真菌进行EST分析，使我们了解了真菌的多样性及其在深海环境中的作用。

Over 300 fungal species were isolated from unique deep-sea environments, including methane cold seeps, 10,000m deep-sea trench, and whale fall sites in this study. Also, many novel unknown fungal groups were revealed in deep-sea environments by analyzing fungal diversity with single cell genomics and next generation sequencer. It was suggested that the group frequently detected from methane cold seep was highly novel and an early diverged group. This study led us to understand fungal diversity and its role in deep-sea environments, through isolating many new and previously unknown species, detecting unknown fungal groups, and using expressed sequence tag (EST) analysis of fungi reported only from deep-sea environments.