Development of accurate electrostatic potential imaging for visualization of protein function

在这项研究中，为了计算出精确的电子密度，我尝试建立了准确的低分辨率衍射数据的测量方法和溶剂区结构的可视化方法。我用高通量X射线构建了一套Spring8的高精度低分辨率数据测量系统。该系统可以获得低分辨率区域的无视差衍射数据。用于显示溶剂区的重原子PbEDTA或Xe可以成功地浸泡在蛋白质晶体中。

In this research, in order to calculate precise electron density, I tried to develop accurate measurement methods of low resolution diffraction data and visualization methods of solvent area structure without error. I constructed a measurement system of precise low resolution data at SPring-8 with high flux X-ray. Los-free diffraction data in low resolution area could be obtained in this system. Heavy atoms, PbEDTA or Xe, for visualization of solvent area could successfully soaked in protein crystal.