The elucidation of the humoral immunity control mechanism by heme

转录抑制因子Bach2具有基本区域亮氨酸拉链（bZip）基元，介导与Maf癌蛋白的二聚体形成和DNA结合。研究发现，血红素在体外抑制Bach2的DNA结合活性。并诱导B细胞中Bach2的降解。为了揭示血红素介导的Bach2调控的分子机制，我们利用光谱分析绘制了血红素结合区。此外，Bach2的各种仪器分析结果表明，Bach2的非结构化区域在血红素结合和/或转导血红素作用中对Bach2的功能调节很重要。

The transcriptional repressor Bach2 possesses the basic region-leucine zipper (bZip) motif that mediates dimer formation with Maf oncoproteins and DNA binding. It has been found that heme inhibits the DNA binding activity of Bach2 in vitro.and induces degradation of Bach2 in B cells. To reveal the molecular mechanism of the heme-mediated regulation of Bach2, we mapped the heme-binding region by using spectroscopic analyses. Moreover, the results of various instrumental analysis of Bach2 revealed that the unstructured region of Bach2 is important for heme binding and/or transducing heme effect upon the functional regulation of Bach2.