Analysis of the factors of host-parasite relationship in severe malaria pathology

1)在PlasmoDB上搜索候选蛋白以确认配体与SRs的匹配。鉴定了其中的4种疟原虫蛋白，并利用大肠杆菌用常规方法成功制备了它们的重组蛋白，目前已证实了它们的生物学功能。2)通过PbANKA （PbANKA）感染小鼠，观察SRs的表达。PbANKA感染后，A类SR （MARCO）在肺、肝和脾的表达明显增强，而B类SR （CD36）的表达不明显。组织病理学上，MARCO在肝窦边缘区巨噬细胞和内皮细胞上表达。采用DNA微阵列技术检测Py17XL （Py17XL）感染致死组和恢复组小鼠的基因表达分化情况。与致死组相比，恢复组59304基因中5893的表达增强。结果表明，在死亡小鼠（M2a巨噬细胞）和恢复小鼠（m2c样巨噬细胞）中均诱导了不同类型的巨噬细胞。

1) To confirm the ligands to SRs, the candidate proteins were searched on the PlasmoDB. Four of the Plasmodiumproteins were identified and their recombinant proteins were successfully made by common methods using E. coli, and now confirming their biological functions. 2) The expression of SRs was observed in mice infected with P. b e r g h e i ANKA (PbANKA). The expression of class A SR, MARCO, was markedly enhanced by PbANKA infection in lung, liver, and spleen, but not class B SR, CD36. MARCO was histopathologically expressed on the macrophages in marginal zone macrophages and endothelial cells in liver sinusoid. The differentiation of gene expression in both lethal and recovered mice group from P. y o e l l i17XL (Py17XL) infection were measuredby DNA microarray. The expression of 5893 in 59304 genes was enhanced in recovered group compared with lethal it. The results suggested that different types of macrophages were induced in both lethal (M2a macrophage) and recovered mice (M2c-like macrophage).