Platelet-derived microparticles augment the adhesion and neovascularization capacities of circulating angiogenic cells obtained from atherosclerotic patients

我们研究了PMPs是否可以促进新生血管的形成。我们从动脉粥样硬化患者的外周血中分离单个核细胞和PMPs，并通过将单个核细胞和PMPs共培养来产生PMP-CACs。PMP-CACs的粘附能力较CACs强。PMP将RANTES释放到培养基中。大鼠后肢缺血后静脉注射PMP-CACs比单纯注射CACs能促进缺血肢体的新生血管形成，PMP-CACs进入缺血肢体毛细血管的数量多于CACs进入缺血肢体毛细血管的数量，PMP-CACs增强的粘附和新生血管形成能力可被RANTES中和抗体所抵消，PMP-CACs增强的粘附和新生血管形成能力可被RANTES中和抗体所抵消。RANTES的分泌可能在增加CACs的粘附和新生血管形成能力中起作用。注射PMP-CACs可能是一种新的策略，以增强动脉粥样硬化患者肢体缺血的治疗性血管生成的效果。

We investigated whether PMPs could augment the neovascularization. We isolated mononuclear cells and PMPs from atherosclerotic patient-derived peripheral blood and generated PMP-pretreated CACs(PMP-CACs) by co-culture of the mononuclear cells and PMPs. The adhesion capacity of PMP-CACs was greater to that of CACs. PMPs released RANTES into the culture medium. Intravenous injection of PMP-CACs to rats with hindlimb ischemia augmented neovascularization of the ischemic limbs more than the injection of CACs.The number of PMP-CACs incorporated into the capillaries of the ischemic limbs was greater than that of incorporated CACs.The augmented adhesion and neovascularization capacities by PMP-CACs were canceled out by a RANTES neutralizing antibody.PMP-secreted RANTES may play role in the augmenting adhesion and neovascularization capacities of CACs. Injection of PMP-CACs may be a new strategy to augment the effects of therapeutic angiogenesis for limb ischemia in atherosclerotic patients.