Identification of the causative antigens of idiopathic steroid-sensitive nephrotic syndrome in childhood by proteome analysis

提示一些体液因素是儿童特发性肾病性类固醇敏感肾病综合征（ISSNS）的病因。本研究旨在确定这些体液因素。由于体液因子成分主要是一种蛋白质，在本研究中，我们使用了一种称为表面增强激光解吸/电离飞行时间质谱法的蛋白质组学分析来鉴定ISSNS患者中高表达的血清蛋白。与缓解期和小儿继发性肾病综合征患者相比，在急性期检测到载脂蛋白C1显著升高。这是首次报道载脂蛋白C1升高与ISSNS病理生理之间的关系，这一发现为进一步研究ISSNS的病理生理提供了一个有趣的起点。

It is suggested that some humoral factors are the causative agents of idiopathic nephrotic steroid-sensitive nephrotic syndrome (ISSNS) in childhood. This study aimed to identify this humoral factors.Since a humoral factor component mainly of a protein, in this study, we used a proteomic analysis called surface-enhanced laser desorption/ionization time of flight mass spctrometry to identify serum proteins which is highly expressed in ISSNS patients. Apolipoprotein C1 was detected at significant elevation during the acute phase compared with the remission phase and pediatric patients with secondry nephrotic syndrome. This is the first report that shows a relationship between an elevation of apolipoprotein C1 and the pathphysiology of ISSNS, and this findings present an interesting starting point for further investigation into the pathophysiology of ISSNS.