Epigenomic analysis and development of therapeutic target for malignant melanoma

为了鉴定黑色素瘤中具有干细胞特征的亚群，我们用荧光激活分选法分离细胞，并将细胞植入NOD/SCID小鼠体内，进行球体形成实验。AKI黑色素瘤细胞中ABCB5阳性亚群和WM266细胞中CD271阳性细胞比CD271阴性细胞和ABCB5阴性细胞更具致瘤性。我们还对慢循环、标记保留细胞进行了分析。标记滞留细胞球体形成率高于其他细胞，标记滞留细胞CD271和ABCB5表达增强。这些结果表明黑色素瘤细胞中的这些亚群具有干细胞特征。我们认为，对这些细胞的表观遗传学变化的额外分析可以用来发现黑色素瘤的新治疗靶点。

To identify subpopulation having stem cell characteristics in melanoma, we isolated cells by fluorescence-activated sorting and implanted cells to NOD/SCID mouse and performed sphere formation assay. ABCB5 positive subpopulation in AKI melanoma cells and CD271 positive cells in WM266 cells were more tumorigenic than CD271 negative cells or ABCB5 negative cells when grafted to mice. We also analyzed slow cycling, label retaining cell. The rate of sphere formation of label retaining cells was higher than the other cells and label retaining cells showed increased expression of CD271 and ABCB5. These result show these subpopulation in melanoma cells have stem cell characteristics. We think additional analysis of epigenetic change of these cells can be used to detect new therapeutic target for melanoma.