Development Research on Single-chain Antibodies against the SmallAntigen through Application of Molecular Evolutionary Engineering

研究表明，晚期糖基化终产物（AGEs）在各种组织中的形成和积累与动脉粥样硬化、糖尿病并发症和衰老相关疾病有关。为了确定ages在各种病理组织中的积累，使用单克隆抗age抗体进行免疫学研究是非常有效的。然而，目前仅有少数几种抗衰老抗体被发现。事实上，由于AGE结构的多样性和异质性以及它们的小分子尺寸，制备具有高亲和力和高特异性的单克隆抗体并不容易。因此，我们采用蛋白工程的方法来开发对各种AGEs具有高亲和力的重组抗体。在本研究中，我们通过噬菌体展示实验制备了针对ga -吡啶的单链抗体（scFvs）

It has been proposed that formation and accumulation of advancedglycation end products (AGEs) in various tissues are involved in atherosclerosis,diabetic complications and aging-associated diseases. In order to determine AGEsaccumulation in various pathological tissues, immunological studies using monoclonalanti-AGE antibodies are highly effective. However, only a few of antibodies against AGEshave been established at present. Actually, it is not easy to prepare monoclonalantibodies that have high-affinity and high-specificity against AGEs, because of thediversity and heterogeneity of AGE structures and their small molecular sizes. Therefore,we have conducted protein-engineering approaches to develop recombinant antibodies withhigh affinity for various kinds of AGEs. In the present study, we have carried out phagedisplay experiments to prepare single-chain antibodies (scFvs) specific for one of AGEstructure, GA-pyridine